SECTION SIX 



14. Turn off the heat and allow the slides to cool in the oven 

 for about thirty minutes. 



15. Take the slides out of the furnace and lay coverslips over 

 the sections, with a pair of forceps. 



16. Seal the coverslips with De Kotchinsky's cement. 



17. De-wax and stain the control serial sections, which have 

 been set aside for the purpose, with suitable stains. 



18. Examine the unstained, incinerated specimens comparing 

 them with control serial sections. This can be most conveniently 

 accomplished by employing two identical microscopes connected 

 with a comparing ocular, the microscope with the normally stained 

 sections being illuminated with ordinary bright-light condenser, 

 and the one with the incinerated sections with a dark-field illumi- 

 nator : this method facilitates the location of the same structure in 

 both types of sections. 



Notes: 



There are many methods, some of which are given in this 

 book {see index) of identifying the numerous minerals which 

 might be present, but it is not possible to give them all here 

 without entering the realms of Mineralogy, Fluorescence Analysis 

 and kindred subjects which are foreign to a book of this kind, but 

 which are well catered for in other text-books to which the reader 

 is referred as well as to the various references given in McLung^s 

 Handbook of Microscopical Technique^ Laboratory Technique in 

 Biology and Medicine, by E. V. Cowdry, and the methods given 

 in Microscopic Histochemistry by G. Gomori. 



3.HYDROXY - 2-NAPHTHOIC ACID - TETRAZOTIZED 



o-ANISIDINE 



For demonstrating sites of carbonyl activity in frozen 



sections 



Solutions required: 



Important : All alcohol used in this technique must be aldehyde free. 



A. 3-hydroxy - 2-naphthionic acid hydrazide o-i gm. 



50% alcohol . . 95 ml. 



Acetic acid glacial 5% . . . . • • 5 ml. 



333 



