MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



8. Wash in tap water until the slide is pale yellow: this should 

 take about fifteen seconds. 



9. Stain in solution D for one to three minutes. 



ID. Rinse with three changes of 70% alcohol for five seconds 

 in each. 



11. Wash for five seconds in 95% alcohol 



12. Wash with acetone for five seconds. 



13. Wash with solution E (acetone-xylol) ; for five seconds. 



14. Wash with three changes of xylol. 



15. Mount synthetic resins; e.g. D.P.X. or Cristalite. 



Results: 



Colloid, cytoplasm and connective tissue elements are stained 

 from light yellow to light brown. Cell nuclei are stained deep- 

 blue to black. 



Sites of radioactivity are indicated by blackening of the photo- 

 graphic emulsion. 



Reference: Simmel, Eva B., Fitzgerald, P. J. and Godwin, J. T. (195 1), 

 Stain Tech., 26, 25-8. 



METHYL - GREEN - PYRONIN - RIBONUCLEASE (Brachet) 



For detecting ribonucleic acid and desoxyribonucleic acid 



in the same cell 



Solutions required: 



I. Zenker's fixative containing 5% acetic acid 

 or 



