MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



End knobs Dark blue 



Middle piece Sheath dark blue, centre dark pink 



Axial filament Dark blue 



Tail Dark blue 



Notes: 



It is suggested that the technique which has been tried out 

 only on dog and human semen, might be applied to other species. 



The technique gives good differentiation and preservation of 

 cytological structure, reliable fixation and staining and undistorted 

 and easily recognizable detail, upon which assays of semen for 

 fertility depend. 



There is a tendency for abnormal forms of spermatozoa to stain 

 more intensely than the normal forms. 



Reference: Casarett, George W. (1953), Stain Tech., 28, no. 3, 125-7. 



ANILINE GENTIAN VIOLET 



A simple and rapid stain for Treponema pallida 



Solutions required: 



A. Aniline gentian violet. 



B. Sodium hydroxide 1% aqueous 50 ml. 

 Absolute alcohol ., .. 1-5 ml. 



C. Alcohol 5%. 



Technique: 



1 . A loopful of serum from the lesion is spread into a film on a 

 slide and dried in air. If an enlarged syphilitic gland is to be 

 examined, 0-5 ml. sterile saline should be injected into the gland; 

 then aspirated with a i-ml. syringe with a 22-gauge needle. 

 Haemoglobin may be removed, if necessary, with distilled water. 



2. Stain with ten drops each of Solutions A, B and C for two 

 minutes, rocking the slide to ensure thorough mixing. 



3. Wash in running tap water for twenty seconds; dry and 

 examine. 



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