SECTION SEVEN 



4. Drain and blot dry; examine under the oil immersion 

 objective. 



Results: 



Heinz bodies are visible as blue granules, the depth of colour 

 depending somewhat on their size and the thickness of the smear. 



Reference: Webster, S. H., Liljegren, E. J. L. and Zimmer, D. J. (1948), 

 Stain Tech., 23, 97. 



DORNER'S SPORE STAIN 



Solutions required: 



A. Carbol fuchsin (Ziehl Neelsen). 



B. Nigrosin, water soluble . . . . 10 gm. 

 Distilled water . . . . . . 100 ml. 



Dissolve by heating, cool, filter and add: 

 Formalin . . . . . . . . 0-5 ml. 



Technique: 



1. A heavy suspension of the culture is made in a test-tube with 

 three drops of distilled water and three drops of carbol fuchsin are 

 then added. Heat on a boiling water bath for ten to fifteen min- 

 utes. 



2. A loopful each of the stained preparation and Solution B are 

 mixed on a slide, spread into a thin smear and dried rapidly ; then 

 examined. 



Results: 



Spores, red. Bodies of bacteria, almost colourless against a dark 

 grey background. 



EHRLICH'S TRIACID STAIN 



For blood smears 



Solution required: 

 Ehrlich's triacid stain. 



2B 359 



