MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



Technique: 



1. Air-dried films on slides or on coverglasses are fixed by heat. 



2. Allow to cool for a few seconds, then flood films with the 

 staining solution and allow the stain to act for five to ten minutes. 



3. Rinse with distilled water until the stain ceases to come out. 



4. Drain off excess water. 



5. Blot and dry thoroughly; mount in Cristalite. 



Results: 



Erythrocytes, orange. Eosinophile granules, bright red. Baso- 

 phile granules, unstained. Neutrophile granules, purple. Lympho- 

 cytes, unstained. Nuclei, pale green. 



EOSIN - GENTIAN VIOLET 

 For basal bodies of cilia 



Solutions required: 



A. Haematoxylin (Ehrlich). 



B. Eosin 0-5% aqueous. 



C. Aniline gentian violet (Stirling). 



D. Lugol's iodine solution. 



E. Aniline oil. . . . . . . . 10 ml. 



Xylol . . . . . . . . 20 ml. 



Technique: 



1. Stain for two minutes in the haematoxylin solution. 



2. Blue and wash in tap water or in lithium carbonate solution. 



3. Stain for one minute in the eosin solution. 



4. Wash with water. 



5. Stain for two hours in the aniline gentian violet. 



6. Wash well in tap water. 



7. Stain in Lugol's iodine for ten to fifteen minutes. 



360 



