SECTION SEVEN 



8. Wash well with tap water; drain and blot dry. 



9. Differentiate in Solution E then wash in several changes of 

 xylol and mount. 



Results: 



Nuclei, dark blue. Basal bodies, purple. 



EOSIN - METHYLENE BLUE - BASIC FUCHSIN 

 A general stain for bacteria, blood and spirochaetae 



Solution required: 



Eosin 2% in absolute alcohol . . 2-5 ml. 



Methylene blue 3% alcoholic . . 7-5 ml. 



Basic fuchsin 10% alcoholic . . 3-0 ml. 



Absolute alcohol . . . . 12-0 ml. 



Distilled water . . . . . . 25*0 ml. 



Mix well. Allow to stand two or three days in a 

 closed vessel; then filter. 



Technique: 



1. Blood and spirochaete smears are allowed to dry in the air 

 before fixation in methyl alcohol. Bacterial smears are fixed by 

 heat in the usual manner. 



2. Stain smears for one minute in the aboVe solution. 



3. Pour off excess stain and wash thoroughly in distilled water. 



4. Drain; blot carefully but thoroughly and examine. 



Results: 



Bacteria — B. typhosus and paratyphoid organisms, pink. 

 B. coli and B. dysenteriae^ lilac. B. diphtheriae, pink with lilac 

 granules and bars. B. influenza^ and Leptothrix unstained but 

 showing lilac bars and granules. Meningicocciy violet to pink. 



Blood: Erythrocytes, pink to orange. Neutrophiles, cytoplasm, 

 pink. Lymphocyte cytoplasm, purple; nuclei, blue. Basophiles, 

 purple to dark blue; nuclei, dark purple to black. Eosinophile 

 granules, pink to opaque white. Polychromatophilic cells, red. 



Spirochaetes : pink to violet. 



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