SECTION SEVEN 



FLAGELLA STAIN (Cesares-Gil) 

 For colon and typhoid organisms 



Solutions required: 



A. Flagella stain (Cesares-Gil). 



B. Carbol fuchsin (Ziehl Neelsen). 



Technique: 



1. A freshly prepared mixture consisting of i vol. of the stain 

 and I vol. distilled water is filtered directly onto unfixed films of a 

 young culture (eighteen to twenty-four hours old) and allowed to 

 act for one minute. 



2. Wash with distilled water. 



3. Stain for five minutes with carbol fuchsin (Ziehl Neelsen), 

 freshly filtered. 



4. Wash with distilled water; blot and dry in air. 



Results: 



Bacteria and flagella, red. Background, very fine granular red 

 precipitate. 



Dissolve; then set aside a few millilitres. The 

 bulk is treated as follows: 



Add ammonia drop by drop, with constant shaking, 

 until the brownish precipitate, which is formed, just 



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