SECTION SEVEN 



Results: 



Flagella are stained violet, as are undulating membranes and 

 cirri of Hyportichs. 



Note: The technique has been found useful for demonstrating 

 the various stages of Dimastogamoeba from amoeboid to flagellat- 

 ing forms after some of a culture has been kept in rain water for 

 one to several hours. 



Reference: Noland, L. E. (1928), Science, 67, 535. 



GIEMSA STAIN 

 For blood, malaria parasites, trypanosomes, etc. 



Note: Best results are obtained by buffering the distilled water 

 to 72. 



Rapid method for films: 



1. Fix air-dried films for three minutes in pure methyl alcohol. 



2. Stain for five minutes in a mixture consisting of one part of 

 Giemsa stain and two parts of distilled water. 



3. Wash with distilled water for one half to one minute; then 

 blot and dry in air. 



Rapid method for spirochaetes : 



1. Air-dried films are fixed by heat by drawing through the 

 flame. 



2. Allow the slide to cool; then flood the slide with a freshly 

 prepared mixture consisting of 10 drops of Giemsa stain to 10 ml. 

 distilled water. 



3. Heat the slide gently till steam rises; allow to cool for about 

 twenty seconds ; then pour off the stain and repeat the process five 

 or six times. 



4. Wash with distilled water; blot dry and mount. 



Slow method for films, for demonstrating spirochaetes, 

 trypanosomes, etc. : 



I. Air-dried films are fixed for three minutes in pure methyl 

 alcohol. 



365 



