MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



HAEMATOXYLIN (Phosphotungstic, Mallory) 



For Entamoeba 



Solutions required: 



A. Absolute alcohol . . . . i volume 

 Mercuric chloride, saturated, 



aqueous . . . . . . 2 volumes 



B. Iodine 1% alcoholic. 



C. Phosphotungstic acid haematoxy- 

 lin (Mallory). 



Technique: 



1 . Thin smears are made on scrupulously clean slides and fixed 

 while still moist in the mercuric chloride solution for fifteen min- 

 utes. 



2. Wash and immerse in water for a few seconds. 



3. Flood slides with the iodine solution and leave thereon for 

 three minutes. 



4. Rinse thoroughly with distilled water until the iodine colour 

 is completely extracted. 



5. Stain for half an hour in phosphotungstic acid haematoxylin. 



6. Rinse in water. 



7. Dehydrate with 95% and absolute alcohol ; clear in xylol and 

 mount. 



Results 



Nuclei, deep blue; cytoplasm, pale blue. 



HAEMATOXYLIN (Weigert)— BORDEAUX RED 

 For permanent preparations of anopheline midgut 



Solutions required: 



A. Absolute alcohol . . • • 45 n^l- 



Formalin .. .. .. 3-5 ml. 



Glacial acetic acid . . . . 1-5 ml. 



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