SECTION SEVEN 



B. Weigert's haematoxylin, A. 



C. Weigert's haematoxylin, B. 



D. Bordeaux Red 0-05% in absolute 



alcohol. 



Technique: 



1 . The specimen together with dissecting medium is placed on a 

 slide and covered with a coverglass ; it is then fixed for seven min- 

 utes by drawing Solution A under the coverglass on one side, 

 whilst the dissecting medium is drawn off on the opposite side 

 with a piece of filter paper. 



2. Flood the slide while the coverglass is still on with Solution 

 A for five minutes. 



3. Very carefully lift the coverglass with a needle so that the 

 gut adheres either to the slide or to the coverglass. 



4. Carefully wash the specimen with 70% alcohol ; then cover 

 it with the alcohol for five minutes. 



5. Cover with 50% alcohol for five minutes. 



6. Stain for thirty seconds with a freshly prepared mixture con- 

 sisting of 0*5 ml. each Solutions of B and C, and 25 ml. 50% alco- 

 hol. 



7. Very carefully wash with 50% alcohol; cover with 70% for 

 five minutes, followed by 95% for fiwt minutes. 



8. Stain for ten seconds in Solution D. 



9. Flush with absolute alcohol, very carefully; clear in xylol 

 and mount. 



HAEMATOXYLIN EOSIN - INDIGO CARMINE 

 For the differential staining of vaginal smears 



Solutions required: 

 A. Haematoxylin (Harris). 



