MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



C. Indigocarmine . . . . . . 0-25 gm. 



Eosin, yellowish . . . . . . 0-75 gm. 



Distilled water . . . . . . i ml. 



Chloroform . . . . • • 5 drops 



Technique: 



1 . Make smears on scrupulously clean slides ; allow to dry. 



2. Immerse in 95% alcohol for three minutes. 



3. Transfer to and immerse in distilled water for one minute. 



4. Transfer to and immerse in haematoxylin (Harris) for twenty 

 minutes. 



5. Wash with running tap water for two minutes. 



6. Immerse in Solution B for a few seconds. 



7. Stain in Solution C over-night. 



8. Rinse quickly in tap water. 



9. Dehydrate with 70%, 90% and absolute alcohol. 



10. Clear in xylol. 



1 1 . Mount in Cristalite. 



Results: 



Nuclei of epithelial cells are stained grey, while the cytoplasm is 

 violet. Cornified cells, brilliant pink. Erythrocytes, pink. 



IODINE - EOSIN 

 For intestinal amoebae and flagellates in faeces 



Solutions required: 



A. Saturated solution of iodine in 5% 



aqueous potassium iodide. 



B. Eosin, yellowish, aqueous 10%. 



C. Equal volumes of Solutions A and B. 



Technique: 



I . Spread a loopful of faeces on a slide with one or two drops of 

 Solution C. 



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