SECTION SEVEN 



2. Mix well and cover with a coverslip before examining under 

 the microscope. 



Results: 



Cysts of amoebae and flagellates are stained yellow to greenish 

 yellow against a red background, while glycogen bodies within 

 the cysts are stained brown. 



JANUS GREEN B 

 For staining oocysts of Coccidia 



Solutions required: 



A. Sodium chloride, saturated, aqueous. 



B. Janus green B aqueous o-oi%. 



C. Eosin, yellowish io% in tap water. 



Technique: 



1. The faecal material is strained through a double layer of 

 cheese muslin. 



2. Centrifuge in ordinary pointed tubes. 



3. Add more water and repeat the process twice, to remove 

 most of the debris. 



4. Add sodium chloride solution, shake well and centrifuge, 

 when the oocysts will appear on the surface. 



5. Collect oocysts and transfer to a slide. 



6. Cover with a coverslip. 



7. Replace the sodium chloride with glacial acetic acid by draw- 

 ing the acid under the coverslip by means of filter paper. 



8. Gently warm the slide for ^lyq. or ten minutes, taking care 

 not to evaporate the acid. 



9. Replace the acid with a freshly prepared solution of janus 

 green B (Solution B above) by drawing the stain under the cover- 

 slip by means of a piece of filter paper; then allow the stain to act 

 for ten minutes. 



10. Wash and stain with the eosin solution for five minutes. 



1 1 . Wash in water. 



2c 375 



