MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



12. Blot off excess water and seal with petroleum jelly or with 

 glycerine jelly. 



Note: The whole of the process of staining and washing can 

 be observed under the low-power objective; but studying is 

 recommended under the oil-immersion objective. 



Results: 



Oocyst jelly is stained red while the walls may appear reddish, 

 and the structures within the sporozites are rendered visible- 

 Abstract Science y 73, 212-13, 1931, H. B. Crough and E. R. Becker. 



JANUS GREEN, B - NEUTRAL RED 

 For supravital staining of blood 



Solutions required: 



A. Janus green, B vital stain 0*4% in neutral 

 absolute alcohol. 



B. Neutral red chloride, vital stain 0-25% in 

 neutral absolute alcohol. 



C. Solution A . . . . . . . . 0-07 ml. 



Solution B . . . . . . . . 1 75 ml. 



Absolute alcohol, neutral . . . . 10 ml. 



Note: Solutions A and B are stable, but solution C deteriorates 

 after a few hours, and it should, therefore, be prepared as and 

 when required, for immediate use. 



Technique: 



1. Scrupulously clean, dry slides are flooded with solution C. 



2. Drain and leave to dry. 



3. Place a small drop of blood on each slide. 



4. Cover the blood with scrupulously clean, dry coverslips and 

 allow the blood to spread. 



5. Seal the edges of the coverslips with soft paraffin wax (M.P. 

 38° C), and examine under the microscope. 



Results: 



Basophilic granules: brilliant scarlet. Eosinophilic granules: 



376 



