SECTION SEVEN 



yellow to light orange. Neutrophilic granules: salmon colour. 

 Mitochondria: small blue dots or rods. Nuclei: unstained. 



Note: The proportion of the two stains may be varied to suit 

 the particular specimen ; for instance, specimens very rich in cells, 

 such as leucaemic blood, need more concentrated mixtures of the 

 stains. 



JENNER STAIN 

 For cytological examination of blood 



Technique : 



1. Air-dried, unfixed blood films are stained for three minutes, 

 face downwards to prevent precipitate depositing on the film and 

 obscuring the picture. 



2. Wash with distilled water until the film appears pink to the 

 naked eye. 



3. Blot dry and examine. 



Results: 



Neutrophile granules, pink. Oxyphile granules, brownish red. 

 Basophile granules, purple. Nucleoli (plasmosomes), pink. Cyto- 

 plasm of partially haemoglobinated precursors of erythrocytes, 

 varying shades of purple. Mature erythrocytes, deep pinkish 

 orange. 



LEISHMAN STAIN 

 For blood, malaria parasites, trypanosomes, etc. 



This stain offers a simple and precise method of staining blood 

 for diagnostic purposes. 



Best results are obtained by buffering the distilled water to pH 

 6-6-70. 



Technique for blood films : 



Fixation is unnecessary unless the films are to be kept in stock 

 for any length of time, in which case they should be fixed for five 

 minutes in pure methyl alcohol at room temperature. 



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