SECTION SEVEN 



Glycerine . . . . . . . . 20 ml. 



Grind together in a mortar and pestle adding: 

 Distilled water . . . . . . 100 ml. 



in portions of about 10 ml. over a period of 

 about 20 minutes until solution is complete; 

 then filter. 



Technique: 



1 . Spread smears on scrupulously clean slides and fix by flaming 

 in the usual way. 



2. Flood slides with the stain and heat till steam rises, then re- 

 move flame and allow the warm stain to act for about 45 seconds. 



3. Pour off excess stain. 



4. Wash with distilled water; then immerse in distilled water 

 for about 30 to 60 seconds. 



5. Shake off excess distilled water; then blot dry and examine. 



Results: 



Gonococci are stained deep red while other bacteria, except 

 those of the Neisseria group, are faint purple or almost unstained. 

 Cytoplasm of pus cells pink while the nuclei are green. 



METHYL VIOLET loB 

 For the direct staining of elementary bodies 



Solutions required: 



A. Methyl violet loB aqueous 1%. 



B. Sodium bicarbonate. . .. .. 2 gm. 



Distilled water (cold) . . . . 100 ml. 



Dissolve by shaking. 



Technique: 



1. Scrupulously clean slides are prepared by rinsing in nitric 

 acid, followed by water ; then alcohol-ether. 



2. Spread films, of a more or less pure suspension, on slides. 

 If much protein is present rinse the films with saline and then with 

 distilled water before allowing to dry in the air or in the incubator. 



3. Fix by flaming in the usual way. 



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