SECTION SEVEN 



SAFRANIN - LIGHT GREEN 

 For spirochaetes, etc. 



100 gm. 

 100 ml. 



7-5 ml. 

 100 ml. 



Solutions required: 



A. Tannic acid 

 Alcohol 95% 



B. Acetic acid, glacial 

 Formalin . . 



C. Safranin i% aqueous. 



D. Light green i% aqueous. 



Technique: 



1. The material suspected of containing spirochaetes is placed 

 in a drop of 5% acetic acid on a slide. The slide is then inverted 

 over the cavity of a well slide and placed in an incubator for fifteen 

 minutes. 



2. Spread the drop with a wire loop and allow it to dry in the air. 



3. Flood the film with a freshly prepared mixture consisting of 

 I volume Solution A and 2 volumes of Solution B and steam from 

 two to five minutes. 



4. Wash with warm distilled water ; then stain with Solution C 

 for four to eight minutes. 



5. Wash with distilled water; counterstain with light green. 



SCHORR'S STAIN 

 For vaginal smears 



Staining solutions: 



A. Harris's haematoxylin. 



B. Biebrich Scarlet . . 

 Orange G 

 Distilled water 

 Glacial acetic acid 



C. Phosphomolybdic acid 

 Phosphotungstic acid 

 Distilled water 



I gm. 

 0*4 gm. 



99 ml. 

 I ml. 



2-5 gm. 

 2-5 gm. 



100 ml. 



395 



