MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



D. Fast Green FCF . . . . . . 0-25 gm. 



Acetic acid, 0-3% . . . . 100 ml. 



Technique: 



1. From the fixing solution, carry through alcohols to water; 

 stain in Solution A for two minutes ; then wash in running water 

 for five minutes. 



2. Stain for one minute in Solution B ; then wash in water. 



3. Mordant in Solution C for one minute; then wash in water. 



4. Stain in Solution D for two minutes ; then without washing : 



5. Differentiate for one minute in 1% acetic acid. 



6. Dehydrate in the usual manner ; clear in xylol and mount in 

 dammar-xylol. 



Note: Solution A may be omitted in certain cases, as in the 

 routine treatment of menopause with oestrogens. With this omis- 

 sion smears can be stained in five minutes. 



Results: 



Cornified cells, orange-red. Non-cornified cells, green. 



SCHORR'S STAIN 



(Single solution) 

 For vaginal smears 



Solutions required : 



A. Harris Haematoxylin 

 B. Schorr's Stain (Single) 

 Technique: 



1. Fix wet smears one to two minutes in equal parts of absolute 

 alcohol and ether : then pass into two changes of absolute alcohol, 

 followed by 80% and 66% alcohol. 



2. Rinse with distilled water for ten seconds; then stain in 

 solution A for two minutes. 



3. Wash in running water for five minutes; then stain with 

 solution B for two minutes. 



4. Dehydrate with 66%, 80% and absolute alcohol; then clear 

 in xylol and mount. 



Results: 



Cornified cells, orange-red. Non-cornified cells, green. 



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