MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



5. Rinse with 70%, followed by 90%, alcohol. 



6. Dehydrate with absolute alcohol. 



7. Clear in xylol and mount. 



Results: 



Comified cells, orange to red. Non-comified cells, blue. 



VAGINAL SMEAR STAIN, PX 

 For vaginal smears 



Technique: 1 



1. Fix smears while still wet in a mixture consisting of equal ' 

 parts absolute alcohol and ether, for two minutes. 



2. Rinse in 70% alcohol. 



3. Rinse in 50% alcohol. 



4. Rinse in water. 



5. Immerse in the staining solution for three minutes. 



6. Immerse in dioxane for a few seconds. 



7. Wash with absolute alcohol rapidly. 



8. Rinse well in xylol and mount. 



Results: 



Nuclei, stained red. Cornified cells, red, pink or orange. 

 Erythrocytes, orange. Basophile cells, blue or green. 



VICTORIA BLUE 4R 

 For elementary bodies 



Solutions required: 



A. Victoria Blue 4R (Lustgarten) . . i gm. 

 Distilled water . . . . . . 90 ml. 



Absolute alcohol . . . . 10 ml. 



B. Potassium hydroxide 0-02% aqueous. 



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