SECTION SEVEN 



Technique: 



1. Smears are spread on scrupulously clean slides and dried in 

 the incubator. 



2. Rinse in physiological saline solution. 



3. Rinse in distilled water. 



4. Fix by immersing in pure methyl alcohol for an hour, in a 

 stoppered jar. 



5. Stain overnight with a freshly prepared and filtered mixture 

 consisting of equal parts of Solutions A and B. 



6. Blot and dry and mount. 



Results: 



Elementary bodies of vaccinia and other viruses are stained dark 

 blue. 



Reference: Gutstein, M. (1937), 7- Path, and Bad., 45, no. i, 313-4- 



VICTORIA BLUE 4R 



For Treponema pallida 



Solution required: 



Victoria Blue 4R (Lustgarten) 

 2% aqueous. 



Technique: 



1. Allow smears to dry in air; then fix with methyl alcohol for 

 ten minutes. 



2. Stain for five minutes in the Victoria blue solution. 



3. Pour off excess stain; rinse with distilled water. 



4. Drain ; dry carefully but thoroughly. 



5. If it is desired to preserve the specimen, mount at once in 

 Cristalite. 



Results: 



Spirochaetes are stained blue ; the characteristic morphology of 

 Treponema pallida clearly distinguishes the organism from other 

 spirochaetes which may be present. 



403 



