MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



PICRIC NITRIC ACID 



Picric acid, aqueous, sat. . . . . • • 95 i^l« 



Nitric acid, cone. . . . . . . • • 5 ml. 



PICRO-SUBLIMATE-ACETIC ACID (RATH'S FLUID) 



Picric acid, saturated, aqueous . . . . 50 ml. 



Mercuric chloride, hot saturated aqueous 50 ml. 

 Glacial acetic acid . . . . . . . . 40 ml. 



PICRO-SUBLIMATE (RAHL'S FLUID) 



Picric acid, saturated aqueous . . . . 50 ml. 



Mercuric chloride, saturated aqueous . . 50 ml. 

 Distilled water . . . . . . . . 100 ml. 



ZENKER-FORMOL 



See Helly's fluid. 



STAINS AND REAGENTS 



ACETO CARMINE (SCHNEIDER) 



Carmine . . . . . . . . . . 0*4 gm. 



Distilled water . . . . . . • • 55 nil. 



Heat to boiling, then add : 



Glacial acetic acid . . . . . . . . 45 ml. 



Raise to boiling point again; then cool and filter. 



ACID FUCHSIN (ALTMANN) 



Aniline water . . . . . . . . 100 ml. 



Acid fuchsin . . . . . . . . 20 gm. 



ACID FUCHSIN (MALLORY) 



Acid Fuchsin . . . . . . . . 0-5 gm. 



Distilled water . . . . . . . . 100 ml. 



ALBERT'S STAIN (for Diphtheria) 



A. 



Toluidine blue 0*3% aqueous . . • • 75 nil. 



Methyl green 0-4% aqueous . . • • 75 nil. 



Glacial acetic acid . . . . . . ..1*5 ml. 



Absolute alcohol . . . . . . • • 4 nil. 



Shake well, heat to about 80° C. then allow to stand for twenty-four 

 hours before filtering. 



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