MEDICAL AND BIOLOGICAL STAINING TECHNIQUES | 



CARBOL METHYLENE BLUE 



Phenol 5% aqueous . . . . . . loo ml. J 



Methylene blue . . . . . . . . i gm. 



Absolute alcohol . . . . . . . . lo gm. 



CARBOL THIONIN 



Thionin (Ehrlich) . . . . . . . . 0*125 g^i* 



Phenol crystals . . . . . . . . i gm. 



Alcohol 10% . . . . . . . . 100 ml. 



Heat to dissolve, or triturate in a mortar; filter when dissolved. 

 The solution deteriorates after a few weeks. 



CARBOL TOLUIDINE BLUE 



Carbol Toluidine blue powder (Michrome 



Brand) . . . . . . . . . . 2-5 gm. 



Distilled water . . . . . . . • 50 ml. 



CARMALUM (MAYER) 



Carminic acid . . . . . . . . i gm. 



Potash alum 5 % • . • . . . . . 200 ml. 



Dissolve by heating. Cool and filter. Add a crystal of thymol or a 

 few drops of chloroform, to the stock bottle, as preservative. 



COTTON BLUE - LACTOPHENOL 



Cotton blue . . . . . . . . i gm. 



Lactophenol. . . . . . . . . . 100 ml. 



COTTON BLUE - MAGENTA LACTOPHENOL 



Cotton blue i % in lactophenol . . . . 8 ml. 



Magenta 1% in lactophenol . . . . 100 ml. 



Lactophenol . . . . . . . . 100 ml. 



DORNER'S NEGATIVE STAIN 



Nigrosin . . . . . . . . . . 10 gm. 



Distilled water . . . . . . . . 100 ml. 



Formalin . . . . . . . . . . 9*5 ml. 



FAST-GREEN FCF IN CELLOSOLVE 



Cellosolve . . . . . . . . . . 100 ml. 



Fast-green FCF . . . . . . • • 3 grfi* 



Heat in a flask on a water bath for about half an hour with occasional 

 stirring; then cool and filter. 



416 



