APPENDIX 



FLAGELLA STAIN MORDANT (LOEFFLER) 



Tannic acid 20% aqueous . . . , 100 ml. 



Ferrous sulphate, cryst. . . . . . . 20 gm. 



Distilled water . . . . . . . . 40 ml. 



Basic fuchsin 10% in absolute alcohol . . 10 ml. 



Dissolve the ferrous sulphate by shaking vigorously v^ith the cold 

 distilled water (do not heat) then add the tannic acid and basic fuchsin. 



GALLOCYANIN (EINARSON) 



Gallocyanin . . . . . . . . 0-3 gm. 



Chrome alum . . . . . . . . 10 gm. 



Distilled water . . . . . . . . 200 ml. 



Boil gently for five minutes; then cool; filter, and make up to the 

 original volume with distilled water. 



GIEMSA STAIN 



Giemsa stain .. .. .. .. 3-8 gm. 



Pure methyl alcohol . . . . . . 250 ml. 



Pure glycerine . . . . . . . . 250 ml. 



Triturate with a mortar and pestle, adding the alcohol and glycerine 

 in small portions at a time during a period of fifteen minutes. Transfer 

 to a stoppered flask and allow to stand for twenty-four hours with 

 occasional shaking; then filter. 



GOODPASTURE'S ACID POLYCHROME METHYLENE BLUE 



Methylene Blue . . . . . . . . 0-25 gm. 



Potassium carbonate 0*25% aqueous . . 100 ml. 



Boil for half an hour, then cool under the tap and add 0*75 ml. 

 glacial acetic acid. 



Shake well then boil gently for five to ten minutes ; cool and make up 

 the volume to 100 ml. with distilled water. 



GRAM'S IODINE 



Iodine pure . . . . . . . . . . i gm. 



Potass, iodide . . . . . . . . 2 gm. 



Water . . . . . . . . . . 300 ml. 



Dissolve the potass, iodide in about 10 ml. of the water; then add the 

 iodine, shake till dissolved ; add the remainder of the water. 



