MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



or: 



Gram's Iodine powder (Michrome Brand) i gm. 



Water . . . . . . . . . . 88 ml. 



Hydrochloric acid, cone. . . . . . . 0.5 ml. 



HAEMALUM (HARRIS) 



Haematoxylin 0-5% aqueous . . . . 100 ml. 



Potash alum . . . . . . • • 5 gni. 



Mercuric chloride powder. . . . . . 0-25 gm. 



Heat to boiling point, then allow to cool, and filter. 



HAEMALUM (MAYER) 



Haematein . • . . . . . . o*i gm. 



Absolute alcohol . . . . . . • • 5 ml* 



Potash alum 5 % aqueous . . . . 100 ml. 



HAEMATOXYLIN (ALUM), AQUEOUS 



Haematoxylin 0-25% aqueous .. .. 400 ml. 



Ammonia or potash alum . . . . 20 gm. 



Thymol . . . . . . . . . . i gm. 



Note: The solution deteriorates after two or three months. 



HAEMATOXYLIN (DELAFIELD) 



Haematoxylin 3*5% in absolute alcohol . . 100 ml. 

 Ammonia alum 6*25% aqueous . . . . 320 ml. 



Glycerine . . . . . . . . . . 80 ml. 



Mix thoroughly and allow to ripen for at least three months; then 

 filter before use. 



HAEMATOXYLIN (EHRLICH) 



Haematoxylin 2% in absolute alcohol . . 100 ml. 



Potash alum 2*5% aqueous .. .. 100 ml. 



Glycerine . . . . . . . . . . 100 ml. 



Glacial acetic acid . . . . . . . . 10 ml. 



Mix well; allow to ripen in a 500-ml. closed bottle for three months 



or: 



Dissolve I gm. Ehrlich haematoxylin powder (Michrome brand) in a 

 mixture of 4 ml. glacial acetic acid, 80 ml. 50% alcohol and 40 ml. 

 glycerine ; then filter. The solution requires no ripening and is ready 

 for immediate use. 



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