APPENDIX 



HAEMATOXYLIN (HARRIS) 



Haematoxylin io% in absolute alcohol . . 5 ml. 



Mercuric oxide . . . . . . . . 0*25 gm. 



Potash alum 10% aqueous . . . . 100 ml. 



Glacial acetic acid . . . . . . • • 4 nil. 



Mix the haematoxylin and alum solutions: raise to boiling point 

 then add the mercuric oxide and when the solution turns deep purple 

 turn off the heat : then cool and add the acetic acid. 



HAEMATOXYLIN (HEIDENHAIN) 



No. I 



Iron alum . . . . . . . . • • 4 gni. 



Distilled water . . . . . . . . 100 ml. 



Dissolve by shaking. Do not heat. 



No. 2 



Haematoxylin 10% in absolute alcohol 



(ripened three to six months) . . • • 5 nil. 



Distilled water . . . . . . • • 95 nnJ, 



HAEMATOXYLIN (IRON) (ANDERSON) 



No. I 



Haematoxylin 10% in absolute alcohol . . 5 ml. 



Absolute alcohol . . . . . . • • 45 nil. 



Distilled water . . . . . . - • 50 ml. 



Calcium hypochloride 2% aqueous . . 3 ml. 



No. 2 



Iron alum 4% aqueous . . . . . . 100 ml. 



Sulphuric acid cone. . . . . • • 3 nil. 



For use, mix two volumes of solution No. i with one volume of No. 

 2; this mixture keeps for a few hours only, and should therefore be 

 prepared only when needed for immediate use. 



HAEMATOXYLIN KULTSCHITZKY 



Haematoxylin 10% alcoholic . . . . 10 ml. 



Acetic acid 2% aqueous . . . . . . 100 ml. 



Dissolve the haematoxylin in the alcohol then shake thoroughly with 

 the acetic acid solution. 



4^9 



