MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



HAEMATOXYLIN PHOSPHOTUNGSTIC MALLORY 



Haematoxylin io% alcoholic . . . . i ml. 





Phosphotungstic acid io% 

 Distilled water 



20 ml. 

 80 ml. 



Ripen by adding 4 drops Hydrogen peroxide (20 volumes) or 5 ml. 

 of 1 % Potassium permanganate. 



HAEMATOXYLIN PHOSPHOMOLYBDIC MALLORY 



Haematoxylin I % aqueous .. .. 100 ml. 



Chloral hydrate . . . . ." . . . 6 gm. 



Phosphomolybdic acid 10% . . . . i ml. 



HAEMATOXYLIN REGAUD 



Haematoxylin 10% in absolute alcohol 



Glycerine 



Distilled water 



10 ml. 

 10 ml. 

 80 ml. 



HAEMATOXYLIN (WEIGERT) 



A. Haematoxylin 10% in absolute alcohol 



(ripened three to six months) . . . . 10 ml. 



Absolute alcohol . . . . . . . . 90 ml. 



B. Ferric chloride (hydrated) 30% aqueous . . 4 ml. 

 Hydrochloric acid . . . . . . . . i ml. 



Distilled water . . . . . . • • 95 nil* 



IODINE GREEN, ACETIC 



Iodine Green i % aqueous . . • • 99 nil. 



Glacial acetic acid . . . . . . . . i ml. 



JENNER STAIN 



Jenner stain powder 

 Pure methyl alcohol 



Dissolve by heating in a flask, lightly plugged with cotton-wool, on 

 a water bath. Allow to cool ; then filter. 



0-5 gm. 

 100 ml. 



LEISHMAN STAIN 



Leishman stain powder 

 Pure methyl alcohol 



Dissolve by heating in a flask, lightly plugged with cotton-wool, on a 

 water bath. Allow to cool; then filter. 



0-15 gm. 

 100 ml. 



420 



