APPENDIX 



LIGHT GREEN IN CELLOSOLVE 



Cellosolve . . . . . . . . . . loo ml. 



Light Green . . . . . . • • 3 gm. 



Heat in a flask on a water bath for about half an hour, with occasional 

 shaking or stirring; then cool and filter. 



LITfflUM CARMINE (Orth) 



Lithium carbonate, saturated aqueous . . loo ml. 



Carmine . . . . . . . . • • 5 giri* 



Boil for fifteen minutes then cool and filter. 



or: 



Lithium Carmine (Michrome Brand) . . 2 gm. 



Distilled water . . . . . . • . 50 ml. 



Heat to boiling point ; allow to cool ; then filter. 



LUGOL'S IODINE 



Iodine pure . . , . . . . . . . i gm. 



Potassium iodide . . . . . . . . 2 gm. 



Water . . . . . . . . . . 100 ml. 



MAGENTA -COTTON BLUE - LACTOPHENOL 



Cotton blue - lactophenol . . . . • • 4 ml- 



Magenta i % aqueous . . . . . . 2 ml. 



Lactophenol. . . . . . . . . • 50 ml. 



MAY-GRUNWALD STAIN 



May-Grunwald stain powder . . . . 0*25 gm. 



Pure methyl alcohol . . . . . . 100 ml. 



Dissolve by heating in a flask, lightly plugged with cotton-wool, on a 

 water bath. Allow to cool, then filter. 



METHYL GREEN - PYRONIN (PAPPENHEIM - UNNA) 



Methyl green .. ... .. .. 0-15 gm. 



Pyronin (B or G) . . . . . . . . 0-25 gm. 



Phenol 0*5% aqueous solution . . . . 80 ml. 



Glycerine . . . . . . . . . . 20 ml. 



Absolute alcohol . . . . . . . . 2-5 ml. 



Dissolve the powder in the phenol solution; then shake vigorously 

 with the glycerine and alcohol : warm to dissolve ; then filter. 



421 



