MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



METHYL VIOLET (JENSEN) 



Methyl violet 6B . . . . . . . . 0*5 gm. 



Distilled water . . . . . . . . looml. 



METHYLENE BLUE (BORAX) 



(MANSON) 



Methylene blue . . . . . . . . 2 gm. 



Borax 5 % aqueous . . . . . . . . 100 ml. 



Note: Methylene blue solutions must not be heated above 60° C. 



METHYLENE BLUE (BORAX) 



(UNNA) 



Methylene blue i gm. 



Borax i % aqueous . . . . . . 100 ml. 



Note: Do not heat borax methylene blue solutions over 60° C. 



METHYLENE BLUE (LOEFFLER) 



Methylene blue . . . . . . • • 3 gni. 



Absolute alcohol . . . . . . • • 30 ml. 



Potass, hydroxide 0'0i% aqueous .. 100 ml. 



METHYLENE BLUE, POLYCHROME (UNNA) 



Methylene blue 

 Potass, carbonate 

 Absolute alcohol 

 Water 



Heat to 60° C. for fifteen minutes. Cool and filter. 



I gm. 

 I gm. 

 20 ml. 

 100 ml. 



or: 



Polychrome methylene blue (Michrome) 3 gm. 

 Distilled water . . . . . . . . 200 gm. 



Absolute alcohol . . . . . . . . 10 ml. 



Dissolve by heating to 60° C and maintain at that temperature for 

 fifteen minutes. Cool and filter; then add the alcohol and mix 

 thoroughly. 



METHYLENE BLUE SULPHURIC (GABBET) ^ 



Distilled water . . . . . . • • 75 ^» 



Sulphuric acid, cone. . . . . • • 25 ml. 



Methylene blue . . . . . . . . 2 gm. 



Pour the sulphuric acid into the water carefully, in a flask, then add 

 the methylene blue and shake or stir to dissolve. 



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