APPENDIX 



MUCICARMINE (MAYER) 



Stock solution: 



Carmine . . . . . . . . . . i gm. 



Aluminium chloride, anhydrous . . . . 0*5 gm. 



Mix thoroughly by grinding to a homogeneous powder in a mortar ; 

 then place the mixture in a flask with 100 ml. of 50% alcohol and boil 

 for two and a half to five minutes on a water bath, shaking the flask 

 at intervals. Cool under the tap at once and filter when cold. 



For use, dilute one volume of this solution with nine volumes distilled 

 water ; 



or: 



Shake 5 gm. of Mucicarmine powder (Michrome Brand) with 200 ml. 

 of 50% Alcohol; then heat to boiling point and continue boiling for 

 2i minutes. Cool; filter. 



MUCIHAEMATEIN 



Mucihaematein powder (Michrome) 



Glycerine 



Distilled water 



0'3 gm. 

 40 ml. 

 60 ml. 



Dissolve by warming and shaking; then cool and filter. 



NEISSER'S STAIN 



A. Methylene blue 

 Absolute alcohol 

 Glacial acetic acid 

 Distilled water 



B. Bismark brown 

 Distilled water 



NEUTRAL RED (JENSEN) 



Neutral red . . 

 I % acetic acid 

 Distilled water 



0'2 gm. 

 4 ml. 

 10 ml. 

 190 ml. 



0*2 gm. 

 100 ml. 



0*1 gm. 

 0*2 ml. 

 100 ml. 



0-175 gm. 

 80 ml. 



NISSL'S METHYLENE BLUE . 



Venetian soap 

 Distilled water 

 Dissolve ; then add : 



Methylene Blue 2% aqueous . . . . 20 ml. 



Shake vigorously then store in a well-closed bottle for at least four 

 months, before use, shaking once or twice a week during this period. 



2F 



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