MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



ORCEIN (UNNA - TANZER) 



Orcein . . . . . . . . . . i gm. 



70% Alcohol . . . . . . . . 100 ml. 



Hydrochloric acid, cone. . . . . . . i ml. 



PARACARMINE (MAYER) 



Carminic acid . . . . . . . . i gm. 



Aluminium chloride, hydrated . . . . 0-5 gm. 



Calcium chloride, anhydrous . . • • 4 gm. 



Alcohol 70% . . . . . . . . 100 ml. 



PHLOXIN - PROPYLENE GLYCOL 



For eosinophile counts 



Phloxin 0*1% in distilled water . . . • 50 ml. 

 Propylene glycol . . . . . . . • 50 ml. 



Stated to give more consistent results with no destruction of eosin- 

 ophiles. 



Reference: Henneman, Wexler and Westenhaver (1949), J. Lab. and Clin. 

 Med., 34, 1017-20. 



PICRO ANILINE BLUE, ALCOHOLIC 



Aniline blue alcohol soluble . . . . i gm. 



70% alcohol. . .. .. .. .. 50 ml. 



Picric acid, 1% in 70% alcohol . . • . 50 ml. 



PICRO ANILINE BLUE, AQUEOUS 



Aniline blue water soluble . . . . o*i gm. 



Picric acid saturated, aqueous . . . . 100 ml. 



PICROCARMINE 



Picrocarmine powder (Michrome Brand) . . i gm. 

 Distilled water . . . . . . . . 50 ml. 



Boil gently for five minutes : allow to cool ; then filter and make up 

 the volume to 50 ml. with distilled water. 



PICRO INDIGO CARMINE 



Picric acid, saturated aqueous . . . . 100 ml. 



Indigo carmine . . . . . . . . 0-25 gm. 



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