APPENDIX 



RANDOLPH'S BLOOD COUNTING FLUID 



Solution A 



Methylene blue . . . . . . . . o-i gm. 



Propylene glycol . . . . . . . . loo ml. 



Solution B 



Eosin, bluish . . . . . . . . o*i gm. 



Distilled water . . . . . . . . loo ml. 



Mix equal volumes of the two solutions and use as a diluting fluid 

 in the white cell pipette. The mixture of the blood and the diluting 

 fluid should remain in the pipette for fifteen minutes before filling the 

 counting chamber. 



Reference: Randolph, T. G. (1943) Proc. S. Exp. Biol. Med., 52, p. 20-2. 



RED CELL COUNTING FLUID (O. T. George) 



Sodium citrate 3% aqueous . . . , 100 ml. 



Formalin (Formaldehyde 40%) . . . . i ml. 



Eosin, bluish , . . . . . . . o-6 gm. 



This solution stains the erythrocytes and causes the cells to lay flat 

 in a single focal plane, thereby facilitating enumeration. 

 Reference: (1954), J'. Lab. and Clin. Med., 40, 479-83. 



SCARLET R, ALKALINE (HERZHEIMER) 



Absolute alcohol . . . . . . • • 35 i^il. 



Distilled water . . . . . . • • 5 ml. 



Sodium hydroxide 10% aqueous . . . . 10 ml. 



Scarlet R . . . . . . . . . . i gm. 



Place in a flask and plug the neck lightly with cotton wool. Heat on 

 a hot water bath until a considerable portion of the dye has gone into 

 solution; then allow to cool before filtering. 



SCHORR'S STAIN 



A. Harris haematoxylin. 



B. Biebrich scarlet . . . . . . . . i gm. 



Orange G . . . . , . . . . . 0-4 gm. 



Distilled water . . . . . . • • 99 ml. 



Glacial acetic acid . . . . . . . . i ml. 



Heat the water: add the stains; stir untill dissolved allow to cool; 

 then add acetic acid ; shake well and filter. 



C. phosphotungstic acid 10% . . • . 25 ml. 

 phosphomolybdic acid 10% . . . • 25 ml. 

 Distilled water . . . . . . . . 100 ml. 



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