MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



D. Fast green FCF aqueous 5 % 

 acetic acid i % 

 Distilled water 



5 ml. 

 32 ml. 

 64 ml. 



SCHORR'S STAIN 

 (Single solution) 



Biebrich scarlet, aqueous 

 Orange G . . 

 Fast green FCF 

 Phosphotungstic acid 

 Phosphomolybdic acid 

 Glacial acetic acid . . 

 Alcohol 50% 



Dissolve by warming ; then allow to cool and filter. 



or: 



Schorr's Stain powder (Michrome) . . i gm. 

 Alcohol 50% .. .. .. .. 55 ml. 



Glacial acetic acid . . . . . . . . 0*5 ml. 



Dissolve the stain by warming with the alcohol ; allow to cool ; then 

 add the acetic acid, shake well and filter. 



i 



TOLUIDINE BLUE (PONDER'S STAIN) FOR DIPHTHERIA 



Toluidine blue 0-02% aqueous . . . . 100 ml. 



Glacial acetic acid . . . . . . . . i ml. 



Alcohol . . . . . . . . • • 5 ml. 



TOLUIDINE BLUE (PUGH) 



Toluidine blue 

 Glacial acetic acid . . 

 Absolute alcohol 

 Distilled water 



REFRACTIVE INDICES 



Absolute alcohol (ethyl) 

 Acetic acid, glacial 

 Acetone . . 

 Aniline oil 

 Aniseed oil 

 Aquamount 

 Balsam tolu 

 Benzene (benzol) 

 Benzyl benzoate 



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