Contents 



Preface 



Part 1 

 Materials and Processes of Slide-Making 



1. Materials and Equipment 3 



Slides and Coverslips — Containers for Handling Objects — Equipment for 

 Handling Slides. 



2. Fixation and Fixatives 6 



The Nature of the Process— Dichromate and Chromate Fixatives— Mercuric 

 Fixatives— Picric Acid Fixatives— Fixatives of Kolmer and Petrunkewitsch 

 —Removal of Fixatives— Softening Hard Objects— Narcotization. 



3. Stains and Staining 15 



The Nature of the Process— Direct Nuclear in Hematoxylin— Indirect Hema- 

 toxylin Staining— Indirect Carmine Nuclear Stains— Synthetic Nuclear and 

 Bacterial Stains— Simple Contrast Stains— Double Contrast Stains— Complex 

 Zoological Staining Techniques— Complex Botanical Staining Techniques- 

 Stains for Special Purposes. 



4. Dehydrating and Clearing 30 



The Nature of the Process— Alcohol Series— Dioxane and Cellosolve— Essen- 

 tial Oils for Clearing — Benzol, Toluol, and Xylol — Necessity for Perfect 

 Dehydration. 



5. Mounts and Mountants 35 



Gum Media— Resinous Media. 



6. Making Wholemounts 39 



Slides for Wholemounts— Cavity Slides— Cells— Mounting Directly in Gum 

 and Resinous Media— Narcotization and Killing— Dehydration and Clear- 

 ing—Mounting in Balsam. 



7. Making Smears 49 



Cleaning Slides— Smearing— Fixing and Staining Smears. 



8. Making Sections , 52 



The Nature of the Process— Free Hand Sections— Paraffin and Sections— 

 Dehydrating Tissues— Clearing Tissues— Choice of an Embedding Medium- 

 Process of Embedding— Preparing the Block— Cutting Sections— Mounting 

 Sections on Slides— Staining and Mounting Sections— Frozen Sections. 



9. Cleaning, Labeling, and Storing Slides 75 



Cleaning and Finishing Gum Mounts — Cleaning and Finishing Resin Mounts 

 —Labels and Labeling— Slide Boxes and Cabinets. 



vii V-H55'' 



