viii CONTENTS 



Part 2 

 Specific Examples of Slide-Making 



1. Preparation of a Wholemount of the Mite in the Medium of Berlese 81 



2. Preparation of a Wholemount of Pectinatella in Balsam after Staining in 

 Grenacher's Alcoholic Borax Carmine 84 



3. Preparation of a Wholemount of a Chicken Embryo in Balsam after Stain- 

 ing in Carazzi's Alum Hematoxylin 89 



4. Preparation of a Wholemount of the Liver Fluke after Staining in Mayer's 

 Carmalum 95 



5. Preparation of a Smear to Show Monocystis in the Seminal Fluid of an Earth- 

 worm Stained in Ehrlich's Acid Alum Hematoxylin 98 



6. Preparation of a Bacterial Smear Stained by the Technique of Lillie 100 



7. Preparation of a Bacterial Film to Demonstrate Gram Positive Organisms. . 102 



8. Demonstration of Tubercle Bacilli in Sputum by the Ziehl-Neelsen Tech- 

 nique 104 



9. Preparation of a Free-hand Section of a Root Stained by the Acid Fuchsin- 

 iodine Green Stain of Chamberlain 106 



10. Preparation of a Section of the Intestine of the Frog Stained in Celestin 

 Blue B-eosin 110 



11. Preparation of a Transverse Section of a Stem of Aristolochia Stained by the 

 Method of Johansen 117 



12. Demonstration of Spermatogenesis in the Testes of the Rat Using Allen's 

 Fixative and Iron Hematoxylin 120 



13. Preparation of a Paraffin Section of the Tongue of a Rat Stained with 

 Celestin Blue B and van Gieson's Picro-fuchsin 125 



14. Preparation of a Paraffin Section of an Amphioxus Using Mallory's Triple 

 Stain 128 



15. Preparation of a Series of Demonstration Slides Each Having Six Typical 

 Transverse Sections of a 72-hour Chick Embryo Stained with Ehrlich's 

 Acid Alum Hematoxylin 131 



Index 135 



