Table 2 (continued) 



Sertions appear 

 opaque or have 

 highly refrac- 

 tive lines out- 

 lining cells 

 and tissues 



1. Clearing agent 

 evaporated before 

 mountant added 



2. Sections insuffi- 

 ciently cleared or 

 cleared in agent 

 not miscible with 

 mountant 



1. None 



1. Obvious 



2. Soak off cover. 

 Clear properly 



2. Check quality and 

 nature of clearing 

 agents and 

 mountants 



Sections will 

 not take stain 

 or stain 

 irregularly 



Wax not per- 

 fectly removed 

 before staining 



2. Section not uni- 

 form thickness 



3. Tissue "old" 

 (has been stored 

 for a long time 

 in alcohol or, 

 worse still, 

 fixative) 



Fixative not suit- 

 able before stain- 

 ing technique 

 employed 



Fixative not fully 

 removed 



1. Return sections 

 through proper 

 sequence of re- 

 agents to xylene. 

 Leave until wax 

 removed. Restain 



2. None 



3. Return sections 

 through proper 

 sequence of re- 

 agents to water. 

 Wash overnight. 

 If not effective, 

 consult Gray's 

 Microtechnique 

 for "tissue 

 revivers" 



4. Try mordanting 

 sections in 

 recommended 

 fixative 



5. See p. 12 for 

 special methods 



1. Change first jar of 

 xylene frequently 



2. See Table 1 



3. Store all tissues 

 embedded in paraffin 

 blocks — never 

 liquids 



4. Obvious 



Treat tissues as 

 indicated on p. 12 



