DOUBLE-STAINED HAND SECTION OF ROOT 109 



Summary 



1. Cut hand sections of plant material hardened and preserved in alcohol. Ac- 

 cumulate sections in water. 



2. Place sections in 1 per cent sodium or potassium hypochlorite until cell 

 contents have been dissolved, leaving clean bleached skeleton. 



3. Wash in water, rinse in 1 per cent acetic acid, and wash again in water. 



4. Stain 24 hours in equal parts 0.2 per cent acid fuchsin and 0.2 per cent 

 iodine green. 



5. Differentiate sections individually in absolute alcohol containing 1 per cent 

 acetic acid and 0.1 per cent iodine. 



6. Transfer directly to terpineol and mount in balsam. 



