116 SPECIFIC EXAMPLES OF SLIDE-MAKING 



2. Wash specimen in running water for some hours. Dehydrate, clear, and 

 embed in wax. 



3. Trim block and attach to microtome. Cut ribbon of sections and mount single 

 sections or short lengths on clean slides, using diluted Mayer's albumen to 

 flatten sections. Dry thoroughly. 



4. Heat dried slides until wax melts. Place in xylene until all wax is removed. 

 Remove xylene with absolute alcohol and transfer slides to water. 



5. Transfer to celestin blue B solution until nuclei are stained black (10 to 15 

 minutes). Wash in water. 



6. Transfer to 1 per cent eosin until sections appear stained sufficiently. Wash 

 in water. 



7. Dehydrate, clear, and mount in balsam. 



