Index 



Acetic acid, contribution to nuclear fixation, 7 

 for differentiating Chamberlain's stain, 108 

 in, Allen's fixative, 1 1 

 Berlese's mountant, 36 

 Bouin's fixative, 10 

 Carnoy and Lebrun's fixative, 10 

 Ehrlich's hematoxylin, 18 

 Gates' fixative, 9 

 Gilson's fixative, 10 

 Heidenhain's fixative, 9 

 Johansen's Fast Green FCF, 28 

 Kolmer's fixative, 1 1 

 Lavdowsky's fixative, 8 

 Navashin's fixative, 9 

 Zenker's fixative, 8 

 Acetone, for dehydration, 44 



in Gray and 'Wess' mountant, 35 

 Acid fiichsin in. Chamberlain's stain, 106 

 Mallory's stain, 25 

 van Gieson's stain, 23 

 Adhesive, Gravis', for frozen sections, 74 



Mayer's, for paraffin sections, 67 

 Agar in. Gravis' adhesive, 74 



temporary wholemounts, 39 

 Alcohol, dehydrating agents for, 34 

 for, Carnoy and Lebrun's fixative, 10 

 Gilson's fixative, 10 

 preserving plant tissues, 106 

 storing wax blocks, 60 

 recommended series of, 30, 55, 70 

 substitutes for, in dehydration, 31 

 Allen's fixative, formula, 1 1 



for rat testis, 120 

 Alum (see Ammonium, Iron, etc.) 

 Ameba, 42 



Ammonium acetate, 12 

 Ammonium alum, 18 

 Ammonium oxalate, 22 

 Amphioxus, recommended fixation, 128 

 staining sections in Mallory's stain, 129 

 treatment after Bouin fixation, 128 

 Anderson's embedding medium, 72, 73 

 Annelida, 43 

 Antiformin, 104 

 Aristolachia, 117-119 

 Arsenous oxide, 35 



Arthropods, collection by Berlese funnel, 81 

 making wholemounts of, 43 



Bacteria, acid fast, 104 

 Gram positive, 102 

 Bacterial smears, preparation of, 

 100, 102, 104 

 staining with, crystal violet, 100, 102 

 magenta, 104 

 methylene blue, 105 

 safranin, 103 

 Balsam, Canada {see Canada balsam) 

 Bark, collecting invertebrates from, 81 



Basic fiachsin {see Magenta) 



Bayberry wax, 56 



Beeswax, 56 



Beetles, 82 



Benzamine hydrochloride, 14 



Benzene, for clearing tissues, 32, 111, 125 



unsuitability for clearing wholemounts, 45 

 Berlese's funnel, 81 

 Berlese's mounting medium, formula, 36 



cleaning from wholemounts, 75 



mounting mites in, 81 

 Bleaching, 97 

 Blood smears, mountant for, 36 



preparation of, 49 



stain for, 27 

 Blueing hematoxylin stains, 17, 93, 133 

 Bone, softening before sectioning, 13 



stains for, 27, 29 

 Borax in, alcohol as preservative, 29 



Grenacher's stain, 19 



stain for bone, 29 

 Bouin's fixative, formula, 10 



softening muscular tissues fixed in, 128 



washing from tissues, 12 

 Box, paper, for embedding, 59 

 Bryozoa, collecting and mounting, 84 



fixation of, 43 



narcotization of, 14, 43, 84 

 Butyl alcohol, 55 



Calcium sulfate, 34 



Camphor, 37 



Canada balsam, alteration of stains by, 124 



cleaning from wholemounts, 45 



discussion of properties of, 37 



finishing slides mounted in, 47, 75 



making wholemounts in, 45 



mounting sections in, 71 



natural, for wholemounts, 88 



with salicylic acid, 130 

 Carazzi's hematoxylin, for chick embryo, 89 



formula for, 18 



in Patay's stain, 26 

 Carbolmagenta, 22 

 Carbolxylene, 32 

 Carmine, as nuclear stain, 19 



Grenacher's alcoholic, 19 



Mayer's alum, 20 



method for liver fluke, 95 



method for Pectinatella, 84 

 Carnoy and Lebrun's fixative, 10 

 Carrot, use in hand sertioning, 53, 106 

 Cartilage, stains for, 26, 27, 29 

 Cedar oil, 55 

 Celestin blue B, as nuclear stain, 21 



method for intestine, 110 



method for tongue, 125 

 Cellosolve, as dehydrating agent, 31 



as stain solvent, 28 



in Hanley's narcotic, 14 



135 



