136 



INDEX 



Cells, for thick wholemounts, 41 

 Celluloid, 41 



Chamberlain's stain, 106, 107 

 Chick embryo, clearing, 93 



differentiating after staining, 93 



embedding, 38 



fitting paper support to, 9 1 



fixative for, 92 



removal from egg, 89 



removal of vitelline membrane from, 90 



sections of, 131 



staining, 92 



storage of, 92 



wholemount of, 89 

 Chitin, softening, 14 

 Chloral hydrate, as narcotic, 43 



in, Berlese's mountant, 36 

 Gray's narcotic, 14 

 Jurray's chitin softener, 14 

 Chloroform, as narcotic, 43 



for clearing, 32 



in Carnoy and Lebrun's fixative, 10 

 Chromic acid, as immobilizing agent, 6 



in, Allen's fixative, 11 

 Gates' fixative, 9 

 lo Bianco's fixative, 9 

 Navashin's fixative, 9 

 Chromosomes, 122 

 Cladocera, 82 

 Cleaning, coverslips, 115 



prepared slides, 75 



slides, before use, 49, 113 

 Clearance angle, 62 

 Clearing, before embedding, 55 



discussion of, 30, 32 



in humid weather, 33 



special techniques, 33 

 Clip, for coverslips, 46 

 Clove oil, as stain solvent, 28 



for clearing, 32 

 Cocaine, 43 

 Cochineal, 15 

 Coelenterata, 14, 42 

 Collembola, 82 

 Connective tissue, 26, 27 

 Conn's crystal violet, 22 

 Contrast stains, 23 

 Coplin jar; 5 

 Copper nitrate, 1 1 

 Copper oleate, 37 

 Copper sulfate, 34, 111 

 Cork, 53 

 Coverslips, applying to sections, 71 



applying to wholemounts, 45, 88 



cleaning, 115 



clip for, 46 



methods of supporting, 41 



types of, 3 



use in flattening specimens, 85 



"Craf ' fixatives, 9 



Crystal violet, for staining bacteria, 100, 102 



formula for Lillie's, 22 

 Crystals in sections, 12 

 Cutting facet, 62 

 Cytoplasmic detail, preservation of, 7 



Decalcification, 12, 13 



Dehydration, before paraffin embedding, 55 



discussion, 30 



Johansen's mixture for, 28 



special jar for. 111 



technique of, 33, 44 

 Dextrin, 72 

 Dextrose, 36 

 Differentiating, chick embryos, 93 



chromosomes, 123 



Ehrlich's hematoxylin, 133 



Grenacher's borax carmine, 87 



iron hematoxylin, 122 



liver fluke, 97 



plant skeletal tissues, 108 



tubercle bacilli, 105 

 Dioxane, for dehydration, 31, 55 



in mountant, 37 

 Dobell's iron hematoxylin, 16 



Earthworm, monocystis in, 98 



Egg albumen, as ribbon adhesive, 67 



in mounting protozoa, 42 

 Eggs, heavily yolked, staining nuclei in, 21 



staining cell walls in, 24 

 Ehrlich's hematoxylin, differentiating, 

 18, 133 



formula for, 18 



staining chick embryo with, 131 

 Elderberry pith, 53 

 Embedding, containers for, 58 



media, Anderson's, 72 

 choice of, 56 

 Hance's, 56 

 Maxwell's, 56 



ovens, 57, 126 



paper box for, 59 



technique for, 57-60 

 Embryo, chick, wholemount of, 89 

 Embryological material, preservation, 

 Embryological watchglass, 4 

 Embryos, staining skeleton in, 29 

 Eosin, as contrast stain, 23 



compound with methylene blue, 27 



ethyl, 23 



staining intestine with, 110 

 Eosin Y, 23 

 Essential oils, dehydrating, 34 



for clearing, 32, 55 

 Ether {see Ethyl ether) 

 Ethyl alcohol {see Alcohol) 

 Ethyl cellosolve, 14 



