INDEX 



139 



Menthol, as narcotic, 43 

 for Bryozoa, 84 

 for liver fluke, 95 

 in Gray's narcotic, 14 

 Mercuric-acetic fixatives, 10, 43 

 Mercuric chloride, as mordant, 128 

 causing crystals in sections, 12 

 in, Gilson's fixative, 10 

 Heidenhain's fixative, 9 

 Helly's fixative, 8 

 Lavdowsky's fixative, 8 

 McNamara, Murphy and Gore's 



decalcifier, 13 

 Zenker's fixative, 8 

 Metaphase figures, 123 

 Methyl alcohol for, dehydration, 44 



fixing smears, 3 1 

 Methyl blue, 25 

 Methyl cellosolve, 22 

 Methyl violet 2B, 28 

 Methylene blue, 105 

 Methylene blue-eosin, 27, 51 

 Microstomum, 43 

 Microtome, adjusting block on, 66 

 choice of, 61 

 for frozen sections, 72 

 knife, 6 1 



action in cutting frozen sections, 72 

 clearance angle, 62 

 cutting angle, 66 

 grinding, 62 

 half ground, 62 

 removing nicks from, 63 

 setting, 62, 63 

 sharpening, 61-63 

 square ground, 62 

 stropping, 64 

 object holders for, 64, 65 

 rotary, 61 

 Schantz, 61 

 Spencer, 61, 65 

 Mites, 81 



Mohr and Wehrle's mountant, 37 

 Monocystis, 98 

 Mordant staining, 15 

 Moss, 81 



Mountants (see also Canada balsam) 

 Berlese's, 36 

 Farrants', 35 

 Gray and Wess', 35 

 gum, 35, 36 

 Mohr and Wehrle's, 37 

 resinous, 41 

 Mounting wax blocks on microtome, 64 

 Muscle, clearing, 125 

 fixing, 125, 128 

 staining, 24, 26, 27 



Narcotic, Gray's, 14 



Hanley's, 14 

 Narcotization, 14, 42 

 Narcotizing, Bryozoa, 84 



invertebrates, 42-44 



liver fluke, 95 

 Navashin's fixative, 9 

 Neelsen's stain, 104 

 Nereis, 43 

 Nervous tissue, 1 1 

 Neutral mountant, 36 

 Nitric acid for, differentiating 



tubercle bacilli, 105 

 in, Gilson's fixative, 10 

 Haug's decalcifier, 13 

 McNamara, Murphy and Gore's 



decalcifier, 13 

 Petrunkewitsch's fixative, 1 1 

 softening Bouin fixed muscles, 128 

 Nuclear detail, preservation of, 7 

 Nuclei {see also Chromosomes) 

 staining, 15 



celestin blue B, 21, 110, 125 

 hematoxylin, 16-19, 120, 131 

 safranin, 22 

 with carmine, 19 



Oil blue N, 29 

 Oil stone, 63 

 Oligochaetae, 43 

 Orange G, 25 

 Orange II, 24 

 Oribatidae, 81 

 Osmic acid, 6, 50, 51 

 Ostracoda, 82 

 Ovens, embedding, 57, 58 

 Oxalic acid, for bleaching, 97 

 in Mallory's stain, 25 



Paper, black, for section identification, 131 

 box, for embedding, 59 

 for supporting chick embryos, 91 

 in fixing liver fluke, 95 

 Paraffin, clearing before embedding in, 55 

 dehydrating before embedding in, 55 

 impregnating tissues with, 57 

 ribbons, attaching to slide, 67 



bubbles in, 69 



cutting, 66 



drying, 68, 69 



ffattening, 67, 69 



frame for protecting, 66 



handling, 66, 67 



identifying sections in, 131 



joining sections to form, 132 



mounting, 67 



mounting individual sections from, 113 



removing plant sections from, 106 



removing wax from, 70 



squeezing to slide, 69 



