140 



INDEX 



Paraffin — (Continued) 



sections, choice of embedding medium 

 for, 36 

 choice of fixative for, 34 

 staining and mounting, 69 

 technique of dehydrating, clearing and 

 embedding, 36 

 Paraldehyde, 37 

 Paramecium, 39, 40 

 Parasitic fungi, 29 

 Parlodion, 74 

 Patay's stain, 26 

 Pectinatella, 84-88 

 Petroleum jelly, 39 



Petrunkewitsch's fixative, for intestine, 1 10 

 for rat tongue, 123 

 formula for, 1 1 

 Phenol, as coupling agent, 32 

 in, Farrants' mountant, 33 

 Jurray's solution, 14 

 Petrunkewitsch's fixative, 12 

 tissue swelling solution, 106 

 Phenyl salicylate, 37 

 Phloroglucinol, 13 

 Phosphomolybdic acid, 26 

 Phosphotungstic acid, 23, 128 

 Picric acid, for differentiating hematoxylin, 16 

 in, Allen's fixative, 1 1 

 Bouin's fixative, 10 

 dehydrating alcohols, 127 

 Smith's stain, 24 

 van Gieson's stain, 23 

 removal from tissues, 12 

 Picro-fuchsin, van Gieson's, 23, 126 

 Picro-spirit blue. Smith's, 24 

 Pipette, 4 

 Pith, 106 



Plant chromosomes, fixation, 9 

 Plant stem, 117 

 Plant tissues, choice of stain 



for wholemounts of, 44 

 reagents for skeletonizing, 107 

 Plasma stains, 23 

 Plaster of Paris, 1 1 1 

 Platyhelminthes, 43 

 Polychaetae, 9, 43 

 Polyvinyl alcohol, 33 

 Ponceau 2R in, Gray's stain, 24 



Patay's stain, 26 

 Potassium alum, as stain diluent, 96 

 in, Carazzi's hematoxylin, 18 

 Mayer's carmine, 20 

 Potassium dichromate, contribution to 

 preservation of shape, 7 

 fixative properties of, 7 

 in, Heidenhain's fixative, 9 

 Helly's fixative, 8 

 Kolmer's fixative, 11 

 Lavdowsky's fixative, 8 

 Zenker's fixative, 8 



Potassium hydroxide, 82 

 Potassium hypochlorite for, hydrolyzing 

 sputum, 104 



skeletonizing plant tissue, 107 

 Potassium permanganate, 97 

 Prothallium, 20 

 Protoplasm, 7 

 Protozoa, fixation of, 42 



smears of, 16 



staining with carmine, 20 

 Pseudoscorpions, 82 

 Pyroxylin, 74 



Quadruple stain, Johansen's, 28 



Radiant heat oven, 37, 126 



Rat, 120 



Regaud's hematoxylin, 16 



Rhizopoda, 42 



Root, 106 



Root tip, 9 



Rope, for collecting Bryozoa, 84 



Rotary microtome, 61 



Rotifera, 14 



Rubber paraffin, Hance's, 36 



Rubber roller, for flattening sections, 69 



Safety razor blade, as microtome knife, 61 



for trimming blocks, 64 

 Safranin, as nuclear stain, 22 



for staining bacteria, 102 



in Johansen's stain, 28, 117-119 

 Salicylic acid balsam, 26, 130 

 Saline, 90, 99 

 Salol, 3 7 



Schantz microtome, 61 

 Scouring powder, 49 

 Sea anemones, 14 

 Section lifter, 4 

 Section of, Amphioxus, 128 



Aristolachia, 117 



frog intestine, 1 10 



rat tongue, 125 



root, 106 



72-hr. chick embryo, 131 

 Section planes, 52 



Sectioning herbarium specimens, 106 

 Sections, for diagnostic purposes, 7 1 



freehand {see Freehand sections) 

 • frozen {see Frozen sections) 



identifying side of slide carrying, 69 



joining to form ribbons, 132 



mounting in balsam, 71 



paraffin {see Paraffin ribbons. Paraffin 

 sections) 

 Setting microtome knives, 62, 63 

 Sharpening microtome knives, 62, 63 

 Shrinking, control of, in fixation, 7 

 Skeleton, animal, 29 



plant, 106 



