1 80 Simon Freed 



DISCUSSION 



Mahler : I can see where this might be useful in the study of the rate of formation of 

 enzyme-substrate complexes. This is a reaction which proceeds much too rapidly to be 

 measured by most ordinary techniques. It is only with very rare and very stable enzyme 

 complexes and by using very interesting and very sensitive experimental devices that Chance*, 

 for instance, has been able to study this at ordinary temperatures. But if one can find the right 

 kind of solvent for both substrate and enzyme — -there is no reason to assume that some of 

 these solvents might not work — one might be able spectroscopically to study the rate of 

 formation of enzyme-substrate complexes at low temperatures. 



* B. Chance and G. R. Williams: The respiratory chain and oxidative phosphorylation. 

 In: Advances in Enzymology, ed. by F. F. Nord 17, 65-134. Interscience, New York. (1956). 



