94 



Biological Stains 



NH, 



N = N /' 



\_/ 



N=N 



CH3 



CH, 



NaSOi NaSOi 



C34H26N606S2Na2; Mol. Wt. 724.710 



{An acid dye; absorption maximum about 497) 

 Solubility at S6°C: in alcohol 0.13% 



This dye has been employed by Zschokke (1888) as a plasma 

 stain especially in contrast to hematoxylin, and by Ono (1934) for 

 staining spirochaetes. 



d41 brilliant PURPURIN R C. I. NO. 454 



NH, 



./ 



BRILLIANT PURPURIN R 



NH, 



-/" 



N=N 



,/\ CH3 CH3 I 



NaSOj NaSOj NaSOj 



C34H25N609S3Na3; Mol. Wt. 826.759 

 {An acid dye) 



Gutstein (1932) has employed a dye which he designates "bril- 

 liant purpur R" as a vital stain for yeasts. It probably is the 

 above. Lillie (1948) includes this dye, with azo fuchsin G and 

 naphthol blue black, in a connective tissue stain. 



d45 vital red C. I. NO. 456 



Synonyms: Brilliant Congo R. Brilliant Congo red R. Acid 

 Congo R. Azidine scarlet R. Brilliant vital red. 



SOjNa 



CH3 



CH, 



N_N 



\ 



\/ \ 



H.N 



x 



SO.N: 



r\=N / \ 



SOiNa 



C34H25N609S3Na3; Mol. Wt. 826.759 

 {An acid dye; absorption maximum about ^98) 



