96 Biological Stains 



D55 TRYPAN BLUE C. I. NO. 477 



Synonyms: Chlorazol blue SB. Benzo blue SB. Dianil blue HSG. 



Congo blue SB. Naphthamine blue SBX. Benzamine 



blue SB. Azidine blue SB. Niagara blue SB. 



H2N OH HO NH2 



\ I N = N_/~V_/~X_N = N 



CH CH 



NaSOa SOaNa ' nIsOs SOsNa 



C34H24N60i4S4Na4; Mol. Wt. 960.808 

 {An acid dye) 



This is a valuable vital stain. By injection into the circulatory 

 system, it is employed for staining the'uriniferous tubules; also for 

 numerous other purposes in the vital staining of various kinds of 

 tissue, from mammals, lower vertebrates and insects. 



d56 EVANS BLUE 



OH NH2 



V~\_/~\_N=N I / 

 \_/ \_/ \./\/\_S03Na 



CH3 CHj ^^y 



SOsNa SOsNa 



C34H24N60i4S4Na4; Mol. Wt. 960.808 



{An acid dye) 



The similarity between Trypan blue and Evans blue is evident 

 by a glance at the two formulae. The latter, like the former, is 

 employed as a vital stain. It has been thus employed in several 

 rather varied applications: see Brunschwig, Schmitz and Jennings 

 (1940), and Zahl and Waters (1941). 



d60 vital NEW RED 



_NH CH, 



N = N I \ O 



\ I H II H |_/ 

 -\N— C— N/ 

 SOjNa NaS03 



C35H28N807S2Na2; Mol. Wt. 782.752 

 {An acid dye) 



No reference can be found to the manufacture of this dye except 

 in England. It is not listed in dye indexes, the dye most nearly 



