Phenyl Methane Dyes 167 



N.55 VICTORIA BLUE 4R C. I. NO. 69O 



Synonym : Fat bine IfR. 



I 

 CH3 CI /— \ N CH3 



CH. \_/-N /-\ 



CH3 

 C34H34N3CI; MoL Wt. 520.093 

 {A basic dye; absorption maxima 593.5 [538.5]) 

 Solubility at 26°C: in water 3.23%; in alcohol 20.^9% 



Herzberg (1934) suggested this dye for staining minute granules 

 in the case of certain virus diseases, claiming these granules to be 

 the filterable virus itself. Others (e.g. Gutstein, 1937) have fol- 

 lowed the same technic, staining the "elementary bodies", but 

 without committing themselves to Herzberg's theory. Isada 

 (1938) mentioned this dye as one of several that may be used in 

 staining bacterial flagella. Lipp (1940) has proposed it, either 

 alone, or mixed with methyl green and pyronin, for staining spiro- 

 chaetes, particularly Treponema pallidum. 



The various Victoria blues are often confused; see also Victoria 

 blue R and B, a few pages below. 



n60 methyl blue C. I. NO. 706 



Synonyms: Cotton blue, Helvetia blue. 



H 



I 

 H /'~\=N_/~\_S03 -Na 



I II \_/ I V-/ 



NaS03_/^\_N_/— \_C SO3 



H 



1 



C37H27N309S3Na2; Mol. Wt. 799.784 

 (An acid dye; absorption maximum about 607) 



On account of the sulfonic groups, this dye is strongly acidic 

 and makes a good counterstain. It may, for instance, be substi- 



