170 Biological Stains 



erythrosin or phloxine in algae; and very widely by pathologists in 

 the Mallory (1900) connective tissue stain, in which it is combined 

 with orange G and acid fuchsin; by Unna in contrast to orcein for 

 staining epithelial sections (see Lee, 1937, p. 438) and in studying 

 the process of chromolysis; by Koneff (1936) in combination with 

 iron hematoxylin as a polychrome staining method for routine 

 laboratory use. 



On account of the lack of constancy in this group, anyone 

 publishing a technic calling for one of these dyes should be very 

 careful to give all the information obtainable from the label and 

 should specify the source from which the sample used was ob- 

 tained. If a Commission certified stain is employed, its certifi- 

 cation number should of course be given. 



PROCEDURES RECOMMENDED BY THE COMMISSION IN WHICH THIS STAIN IS USED 



n70 ISAMINE blue C. I. NO. 7IO (?) 



Occasional reference is made, especially in the European litera- 

 ture, to a dye called isamine blue. Its exact chemical nature is 

 not at present known, although the Colour Index Hsts isamine blue 

 6B or 8B as a synonym of No. 710 (alkah blue XG) and isamine 

 blue B and R as similar to this dye. The dye is not yet manu- 

 factured in America but was available from German sources be- 

 fore the second World War. It is known to be an acid dye, a 

 sulfonated naphthyl-rosanilin or naphthyl-pararosanilin. 



Isamine blue is employed to some extent in Europe as a vital 

 dye by intravenous injection, followed by lead salts or radiation. 

 Exact references to such uses, however, are not now available. 

 Dean (1937) employs it for determining the proportion of antigen 

 to antibody in immune sera. 



n75 patent blue V c. I. NO. 711 



Synonyms: Aljphazurine 2G. Patent blue VF. Pontacyl 



brilliant blue V. 



