174 Biological Stains 



Some Victoria blue, possibly this or the following or possibly 

 C. I. No. 690, has been employed by Gutstein (1932) as a vital 

 stain for yeast, by Hausdorff (1927) for staining spermatazoa in 

 the testicle, and by Ono (1934) for staining spirochaetes in blood. 

 The various Victoria blues are frequently confused in the litera- 

 ture. 



plO VICTORIA BLUE B C. I. NO. 72.9 



Synonyms: Fat blue B. Com blue BN. 



CHj 



/ 

 CH3 CI /~\_N_CH3 



\1 /\ / 



/ \_/ \/ \_NH_ 



CH, 



/ \ 



C33H32N3CI; Mol. Wt. 506.067 

 {A basic dye; absorption maxima 619.2 [567]) 



This dye has been employed by Proescher (1934) for staining 

 neuroglia, and its eosinate by Geschickter (1930a) for fresh 

 frozen tissue. 



Pll NIGHT BLUE C. I. NO. 73 I 



CH2' CH3 



-\_N/ 



/ \CH2.CH3 



HN— \_/ I|/-X=N_CH2.CH3 



CI CH2.CH3 



C37H40N3CI; Mol. Wt. 562.171 



(A basic dye) 



Hallberg (1946) recommended this dye in place of carbol fuchsin 

 as a stain for acid fast bacteria, with phenolic pyronin as a counter- 

 stain. The procedure has been followed to some extent in Europe, 

 but is not much used in America. The dye has recently become 

 hard to obtain commercially. 



