Miscellaneous Dyes 229 



E20 ACID ALIZARIN BLUE GR C. I. NO. IO48 



NH, O OH 



HO I J I I SOjNu 



NaS03/\/\/\/\0H 



HO O NH. 



CuHgNzOi 282X32; Mol. Wt. 506.334 

 (An acid dye) 



Some acid alizarin blue, probably either this or the following, 

 has been employed by Buzaglo (1934) in combination with orcein 

 and gallocyanin as a tissue stain. Peterson (1924) has recom- 

 mended acid alizarin blue in a modification of the Mallory con- 

 nective tissue stain; the procedure seems to be well worth recom- 

 mending, but there proves to be some difficulty in standardizing 

 this dye for the purpose. 



For the acid alizarin blue modification of the Mallory technic, see Staining Pro- 

 cedures, p. IB3-6, 8. 



E«5 ACID ALIZARIN BLUE BB C. I. NO. I063 



Synonyms: Anthracene blue SIVX, SWX extra 



HO O OH 



NaSO, I It I OH 



HO 1 II I S03Na 

 HO O OH 



CuHeOi 482X32; Mol. Wt. 508.302 

 (An acid dye) 



This dye has come into use in connection with fluorescence 

 microscopy, because of the color which it imparts to various tissue 

 elements under ultra-violet illumination. Some anthracene blue 

 has been called for occasionally in aqueous solution with aluminium 

 sulfate. There are, however, two or three other anthracene blues 

 besides this dye; so it is not certain whether this is the one called 

 for in the technic in question. 



e30 ALKANET 



A natural dye of the anthraquinone group, closely related to 

 alizarin. It has rarely been specified for biological use, but Artsch- 

 wager (1943) has employed it as a stain for rubber in plant sections. 



