APPENDIX I 

 TABLES RELATING TO STAINS 



How TO Use These Tables 



Identifying a dye from its index number. Dyes in England and the United States 

 are generally designated by their Colour Index number, in Germany and several 

 other countries by their Schultz number. If the C. I. number alone is known for 

 one of the biological stains, it can be identified by Table 1, in which the dyes are 

 arranged in the order of the C. I. nmnbers; if known by the Schultz number it can 

 be identified by Table 2, in which the Schultz numbers of these same stains are 

 arranged serially, followed by the corresponding C. I. numbers and page references 

 to where the dyes are described in this book. 



Table 1 can also be used in studying the synonymy of the stains; but will not 

 prove as convenient as the general index for identifying a dye from some synonym, 

 as the arrangement is not alphabetical. 



Learning the common uses of stains. In Table 3 the dyes are listed under their 

 preferred designations in the order in which they occur in the main part of this 

 book. In the second column are given their principle uses as stains. References 

 to the authors of the procedures are not given in this table, but can be obtained by 

 consulting the page in the main part of the book where the dye in question is 

 described. 



Selecting stains for some general field of work. Table 5 includes lists of the dyes 

 most commonly used in general fields of microscopic work, e.g. animal histology, 

 plant histology, etc. It should prove useful in selecting the stains needed in 

 equipping a new laboratory in some one of these fields. 



Learning the solubility of a stain. In making solutions of stains it is often de- 

 sirable to know their solubility in either water or 95% alcohol. Figures indicating 

 these solubilities of recrystallized dyes are given in Table 6, which includes all the 

 most commonly used stains, and numerous others; the arrangement in this table 

 is alphabetical. The solubilities of commercial dyes, however, vary widely from 

 these figures for the purified products; the former are ordinarily less soluble, though 

 occasionally the opposite. A limited amount of information is available, thanks 

 to the cooperation of the American Pharmaceutical Association, as to the solubil- 

 ities of commercial samples of about 25 of the commonly used stains; this is given 

 in Table 7, and in some cases may prove more useful in making up a staining fluid 

 than that given in Table 6. 



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