APPENDIX III 

 GENERAL LABORATORY INFORMATION 



1. Solubility of Miscellaneous Compounds Used in 

 Microscopic Work.* 



2. Formulae of Fixing Solutions 



1) Formol: 



Formalin (40% formaldehyde) 4-10 parts 



Dist. water 100 parts 



2) Mercuric chloride: 



Sat. Aqu. (or Ale.) Sol. mercuric chloride 

 5% Glacial acetic acid. 



The proportions of these two solutions to mix together must be determined 

 experimentally in any instance, employing enough of the acetic acid to over- 

 come the shrinking action of the sublimate. 



3) Gilson's fluid. 



Nitric acid, 46° strength (about an 80% solution) .^ ?c. 



Glacial acetic acid 4 cc. 



60% alcohol 100 00. 



Distilled water 880 cc. 



Mercuric chloride 20 g. 



4) Sublimate acetic: 



Sat. Aqu. Sol. of mercuric chloride 95 parts 



Glacial acetic acid 5 parts 



5) Mueller's fluid: 



Potassium bichromate 25 g. 



Sodium sulfate 10 g. 



Dist. water 1000 cc. 



*The data given in this table were obtained from various sources in the literature, 

 and their accuracy is not vouched for; they are, however, sufficiently correct to 

 guide anyone in making up a saturated solution of any of the above compounds. 



QOQ 



