330 Biological Stains 



6) Zenker's fluid: 



Potassium bichromate 2.5 g. 



Mercuric chloride 5-8 g. 



Dist. water ad 100 cc. 



Glacial acetic acid 5 cc. 



Dissolve the mercuric chloride and potassium bichromate in the water with the 

 aid of heat. Do not add the acetic acid to the stock solution, but only to the 

 part taken for hardening pieces of tissue. 



7) Helly's fluid, or Zenker-f ormol : 



This is a modification of Zenker in which the acetic acid is replaced by 5% for- 

 malin. 



8) Carnoy's fluid: 



Glacial acetic acid 1 part 



Absolute alcohol 6 parts 



Chloroform 3 parts 



9) Formol-nitric: 



Formol, 10% 3 parts 



Nitric acid, 10% 1 part 



10) Flemming's fluid. Strong formula. 



(a) Chromic acid, 1% Aqu. Sol 15 parts 



Glacial acetic acid 1 part 



Distilled water 4 parts 



(b) Osmic acid, 2% in 1% chromic acid solution. 



Just before using mix 4 parts of (a) with one part of (b). Use 10 times the 

 volume of the object. 



11) Bichromate-chromic-osmic acid mixture of Champy: 



3% Aqu. Sol. of bichromate of potash 7 parts 



1% chromic acid Aqu. Sol 7 parts 



Osmic acid, 2% Aqu. Sol 4 parts 



12) Platino-aceto-osmic acid mixture of Hermann: 



Platinic chloride, 1% Aqu. Sol 15 parts 



Glacial acetic acid 1 part 



Osmic acid, 2% Aqu. Sol 2 parts 



13) Merkel's fluid (F. E. V. Smith's modification): 



Acetic acid, 5% Aqu. Sol 100 cc. 



Platinic chloride, 1% Aqu. Sol 5 cc. 



Chromic acid, 1% Aqu. Sol 10 cc. 



14) Bouin's fluid: 



Sat. Aqu. Sol. picric acid 75 parts 



Formol, C. P 25 parts 



Acetic acid, glacial 5 parts 



15) Allen's fluid P. F. A.j: 



Picric acid. Sat. Aqu. Sol 75 parts 



Formalin, C. P 15 parts 



Glacial acetic acid 10 parts 



Urea 1 part 



16) Farmer's: 



Absolute alcohol 3 parts 



Glacial acetic acid 1 part 



